Slunga-Tallberg A, Wessman M, Ylinen K, von Koskull H, Knuutila S
Department of Medical Genetics, University of Helsinki, Finland.
Fetal Diagn Ther. 1994 Sep-Oct;9(5):291-5. doi: 10.1159/000263950.
The presence of nucleated erythrocytes was studied before and after enrichment with immunomagnetic beads and monoclonal antiglycophorin A (anti-GPA) antibody in the peripheral venous blood of 11 pregnant women at 10-16 weeks of gestation. Nucleated erythrocytes were identified by alkaline phosphatase antialkaline phosphatase immunostaining by means of anti-GPA antibody. In the unenriched cell samples, nucleated erythrocytes were found at frequencies of 1/2,800-1/83,000 in 6 cases. The frequency of nucleated erythrocytes was increased up to 6 times by the enrichment.
对11名妊娠10 - 16周孕妇外周静脉血中，用免疫磁珠和单克隆抗血型糖蛋白A（抗GPA）抗体富集前后有核红细胞的存在情况进行了研究。通过抗GPA抗体，采用碱性磷酸酶抗碱性磷酸酶免疫染色法鉴定有核红细胞。在未富集的细胞样本中，6例中有核红细胞的出现频率为1/2800 - 1/83000。富集后有核红细胞的频率增加了6倍。
