Bianchi D W, Zickwolf G K, Yih M C, Flint A F, Geifman O H, Erikson M S, Williams J M
Division of Genetics, Children's Hospital, Boston, MA.
Prenat Diagn. 1993 Apr;13(4):293-300. doi: 10.1002/pd.1970130408.
Fetal nucleated erythrocytes (NRBC) in maternal blood are a non-invasive source of fetal DNA for prenatal genetic screening. We compared the effectiveness of three monoclonal antibodies for the separation of fetal cells from maternal blood by flow sorting. Mononuclear blood cells from 49 healthy pregnant women were incubated with antibody to CD 71, CD 36, and/or glycophorin A (GPA), employed singly or in combination with each other. These monoclonal antibodies recognize surface antigens on haematopoietic precursor cells. Successful isolation of fetal cells was defined as detection of Y chromosomal sequences in maternal blood from women carrying male fetuses, with absence of Y sequences when female fetuses were carried. Thus, gender prediction accuracy was used as a measure of fetal cell separation. Using anti-CD 71 to isolate fetal cells, gender prediction was 57 per cent correct; with anti-CD 36, it was 88 per cent correct. Anti-GPA, an erythrocyte-specific antigen, used alone or in combination with anti-CD 71 or 36, improved gender prediction to 100 per cent. We conclude that antibody to GPA improves the retrieval of fetal NRBC from maternal blood, permitting genetic analysis by the polymerase chain reaction.
母体血液中的胎儿有核红细胞（NRBC）是用于产前基因筛查的无创胎儿DNA来源。我们比较了三种单克隆抗体通过流式分选从母体血液中分离胎儿细胞的效果。将49名健康孕妇的单个核血细胞与抗CD 71、抗CD 36和/或血型糖蛋白A（GPA）抗体单独或相互组合孵育。这些单克隆抗体识别造血前体细胞上的表面抗原。成功分离胎儿细胞的定义为：在怀有男性胎儿的孕妇母体血液中检测到Y染色体序列，而怀有女性胎儿时则未检测到Y序列。因此，性别预测准确率被用作胎儿细胞分离的衡量标准。使用抗CD 71分离胎儿细胞时，性别预测准确率为57%；使用抗CD 36时，准确率为88%。抗GPA（一种红细胞特异性抗原）单独使用或与抗CD 71或抗CD 36联合使用时，可将性别预测准确率提高到100%。我们得出结论，抗GPA抗体可提高从母体血液中获取胎儿NRBC的效率，从而允许通过聚合酶链反应进行基因分析。
