Diernaes L, Sehested J, Møller P D, Skadhauge E
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg, Copenhagen, Denmark.
Exp Physiol. 1994 Sep;79(5):755-62. doi: 10.1113/expphysiol.1994.sp003805.
Isolated mucosal sheets of cattle rumen were studied in vitro in Ussing chambers using a computer-controlled voltage clamp. Unidirectional fluxes of Na+ and Cl- were measured in standard (short-chain fatty acid (SCFA)-free) or SCFA-containing Ringer solution under zero voltage clamp conditions. Net NaCl absorption was observed under zero voltage clamp conditions, was stimulated by SCFAs and was not reduced by 1 mmol l-1 amiloride, although short-circuit current was significantly reduced by this concentration of the blocker. A constant coupling ratio of Na+ and Cl- absorption was observed.
使用计算机控制的电压钳在尤斯灌流小室中对牛瘤胃的离体黏膜片进行了体外研究。在零电压钳条件下，于标准（不含短链脂肪酸（SCFA））或含SCFA的林格溶液中测量Na⁺和Cl⁻的单向通量。在零电压钳条件下观察到了净NaCl吸收，其受到SCFAs的刺激，且不受1 mmol l⁻¹氨氯吡脒的抑制，尽管该浓度的阻滞剂显著降低了短路电流。观察到Na⁺和Cl⁻吸收的恒定耦合比。
