Fukushima M, Watanabe S, Kushima K
Tohoku J Exp Med. 1976 Jun;119(2):115-22. doi: 10.1620/tjem.119.115.
Attempts were made to purify the LH-releasing substance extracted from the leaves of Avena sativa by means of two-step chromatographic procedures using a weakly acidic ion-exchange resin (CG-50,type II) and DEAE-Sephadex A-25 (coarse) with successful results. For preliminary fractionation of such starting materials as dried leaves, fresh leaves, and acetone-extracted powder(crude extracts), 5% acetate-buffered active carbon proved to be more effective than starch zone electrophoresis. From its behavior on chromatography with weakly acidic ion-exchange resins as well as Sephadex gel filtration, the active fraction extracted from the leaves of Avena saliva was assumed to be different from the LH-RH present in the hypothalamus. This partially purified material, however, was demonstrated to have an LH-releasing activity by the ovarian ascorbic acid depletion method using Wistar-Imamichi strain rats. Evidence was presented that its site of action is in the adenohypophysis.
人们尝试通过两步色谱法，使用弱酸性离子交换树脂（CG - 50，II型）和DEAE - 葡聚糖A - 25（粗型）来纯化从燕麦叶中提取的促黄体生成素释放物质，并取得了成功。对于干燥叶片、新鲜叶片和丙酮提取物粉末（粗提取物）等起始原料的初步分级分离，5%醋酸盐缓冲活性炭被证明比淀粉区带电泳更有效。根据其在弱酸性离子交换树脂色谱以及葡聚糖凝胶过滤中的行为，推测从燕麦叶中提取的活性部分与下丘脑存在的促黄体生成素释放激素不同。然而，通过使用Wistar - Imamichi品系大鼠的卵巢抗坏血酸耗竭法，证明这种部分纯化的物质具有促黄体生成素释放活性。有证据表明其作用部位在腺垂体。
