[Detection of nitric oxide formed from L-arginine in the murine stomach in vivo by EPR].

Suspension of baker's yeast loaded with a specific trap of nitric oxide (NO), a complex of Fe2+ with diethyldithiocarbamate (DETC), was used for the detection of NO formed in mouse stomach at its adaptive relaxation or under action of ethanol in vivo. NO formation was determined by the increase of intensity of the EPR signal due to mononitrosyl iron complex (MNIC) with DETC which appeared in yeast cells infused into the stomach. An increase of signal intensity was observed in stomach preparations isolated from mice when the doses of yeast suspensions injected p/o into mouse stomach for 40 min increased up to 1.5 ml or when 25% ethanol solutions were added p/o to the stomach. These effects were attenuated when NO-synthase inhibitor, NG-nitro-L-arginine was i/p injected into mice.