Mikoyan V D, Kubrina L N, Serezhenkov V A, Stukan R A, Vanin A F
Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russian Federation.
Biochim Biophys Acta. 1997 Aug 29;1336(2):225-34. doi: 10.1016/s0304-4165(97)00032-9.
In EPR experiments on mice it was demonstrated that a hydrophobic complex Fe2+ with diethyldithiocarbamate (DETC) is a more efficient selective NO trap than a hydrophilic complex Fe2+ with N-methyl-D-glutamine dithiocarbamate (MGD). This difference can be due to the higher stability of paramagnetic nitrosyl iron complex with DETC (MNIC-DETC) formed by NO binding to Fe2+-DETC in animal tissues in vivo. The complex analogue MNIC-MGD is reversibly oxidized in animal blood to transform into the diamagnetic EPR-silent form. The latter is detectable also in urine of animals, especially of those treated with bacterial lipopolysaccharide which initiates the enhanced NO production in the organism. We suggest that NO2 or peroxynitrite formed from endogenous NO can serve as an agent reversibly oxidizing MNIC-MGD in these animals.
在对小鼠进行的电子顺磁共振（EPR）实验中表明，二乙基二硫代氨基甲酸盐（DETC）与Fe²⁺形成的疏水配合物比N - 甲基 - D - 谷氨酰胺二硫代氨基甲酸盐（MGD）与Fe²⁺形成的亲水配合物是更有效的选择性NO捕获剂。这种差异可能是由于在动物组织体内，NO与Fe²⁺ - DETC结合形成的顺磁性亚硝酰铁配合物（MNIC - DETC）具有更高的稳定性。配合物类似物MNIC - MGD在动物血液中被可逆地氧化，转化为抗磁性的EPR沉默形式。后者在动物尿液中也可检测到，特别是在用细菌脂多糖处理过的动物尿液中，细菌脂多糖会引发机体中NO生成增加。我们认为，由内源性NO形成的NO₂或过氧亚硝酸盐可作为一种试剂，在这些动物体内使MNIC - MGD发生可逆氧化。
