Ehninger G, Proksch B, Schiller E
J Chromatogr. 1985 Jul 12;342(1):119-27. doi: 10.1016/s0378-4347(00)84494-1.
A high-performance liquid chromatographic method using ion-pair chromatography on reversed-phase C18 material was developed. After sample clean-up on XAD columns, mitoxantrone at concentrations below 1 ng/ml in serum and 0.2 ng/ml in urine were measurable with a coefficient of variation of less than 9.3% at a wavelength of 658 nm. Four metabolites were separated in urine. The two major metabolites co-chromatographed with the synthesized mono- and dicarboxylic acid derivatives of mitoxantrone. The method allowed the measurement of mitoxantrone and its metabolites in serum up to more than one week and in urine up to four weeks after administration of the drug.
开发了一种在反相C18材料上使用离子对色谱的高效液相色谱方法。在XAD柱上对样品进行净化后，在波长658nm处，血清中米托蒽醌浓度低于1ng/ml、尿液中低于0.2ng/ml时可被检测到，变异系数小于9.3%。在尿液中分离出了四种代谢物。两种主要代谢物与米托蒽醌合成的单羧酸和二羧酸衍生物共色谱。该方法能够在给药后长达一周以上的时间内测定血清中的米托蒽醌及其代谢物，在给药后长达四周的时间内测定尿液中的米托蒽醌及其代谢物。
