DiTella M, Feiguin F, Morfini G, Cáceres A
Instituto de Investigacion Médica Mercedes y Martin Ferreira, Córdoba, Argentina.
Cell Motil Cytoskeleton. 1994;29(2):117-30. doi: 10.1002/cm.970290204.
We report here a novel intracellular localization and function of Tau proteins in cultured cerebellar neurons. Immunofluorescence staining of detergent-extracted cytoskeletons with antibodies specific for Tau proteins revealed intense labeling of growth cone microtubules. Besides, suppression of Tau by antisense oligonucleotide treatment results in the complete disappearance of antigen 13H9, a specific growth cone component with properties of microfilament- and microtubule-associated protein [Goslin et al., 1989: J. Cell Biol. 109:1621-1631], from its normal intracellular location. This phenomenon is unique to neurite-bearing cells, is not associated with the disappearance of microtubules from growth cones, and is not reversed by taxol, a microtubule-stabilizing agent. In addition, Tau-suppressed neurons display a significant reduction in growth cone area and fillopodial number; on the contrary, fillopodial length increases significantly. The alterations in growth cone morphology are accompanied by considerable changes in the phalloidin staining of assembled actin. Taken together, the present results suggest that in developing neurons Tau proteins participate in mediating interactions between elements of the growth cone cytoskeleton important for maintaining the normal structural organization of this neuritic domain.
我们在此报告Tau蛋白在培养的小脑神经元中的一种新的细胞内定位和功能。用针对Tau蛋白的特异性抗体对去污剂提取的细胞骨架进行免疫荧光染色，结果显示生长锥微管有强烈的标记。此外，用反义寡核苷酸处理抑制Tau蛋白会导致抗原13H9（一种具有微丝和微管相关蛋白特性的特异性生长锥成分）[戈斯林等人，1989年：《细胞生物学杂志》109:1621 - 1631]从其正常细胞内位置完全消失。这种现象在有神经突的细胞中是独特的，与生长锥中微管的消失无关，并且不能被微管稳定剂紫杉醇逆转。此外，Tau蛋白被抑制的神经元生长锥面积和丝状伪足数量显著减少；相反，丝状伪足长度显著增加。生长锥形态的改变伴随着组装肌动蛋白的鬼笔环肽染色的相当大变化。综上所述，目前的结果表明，在发育中的神经元中，Tau蛋白参与介导生长锥细胞骨架元件之间的相互作用，这对于维持这个神经突区域的正常结构组织很重要。
