Herrera J L, Vigneulle R M, Gage T, MacVittie T J, Nold J B, Dubois A
Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20889-5145.
Dig Dis Sci. 1995 Jan;40(1):211-8. doi: 10.1007/BF02063968.
Radiation with doses > 7.5 Gy damages the canine intestinal mucosa, and pretreatment with WR2721 reduces this damage. However, the effects of radiation and of WR2721 on in vivo intestinal transport are unclear. Therefore, we determined canine survival, intestinal transport, and mucosal histology following unilateral abdominal irradiation. Isoperistaltic ileostomies were prepared in 23 dogs under general anesthesia and aseptic conditions. After a three-week recovery period, animals were given either placebo or WR2721, 150 mg/kg intravenously, 30 min prior to 10 Gy cobalt-60 abdominal irradiation. Ileal transport and histology were determined in both groups before exposure and one, four, and seven days after irradiation. Seven-day survival was significantly improved by pretreatment with WR2721 (91% vs 33%, P < 0.02). On day 4, both mucosal integrity and net intestinal absorption were significantly better (P < 0.05) after WR2721 than after placebo. Thus, radiation-induced damage to the ileal mucosa is accompanied by a reduction in net ileal absorption of water and electrolytes in vivo. In addition, pretreatment with WR2721 improves animal survival while reducing ileal damage and improving intestinal absorption.
剂量大于7.5 Gy的辐射会损伤犬类肠黏膜，而WR2721预处理可减轻这种损伤。然而，辐射及WR2721对体内肠道转运的影响尚不清楚。因此，我们测定了单侧腹部照射后犬类的存活率、肠道转运及黏膜组织学情况。在全身麻醉和无菌条件下，为23只犬制备了等蠕动回肠造口术。经过三周的恢复期后，在10 Gy钴-60腹部照射前30分钟，给动物静脉注射150 mg/kg的安慰剂或WR2721。在照射前以及照射后1天、4天和7天，测定两组动物的回肠转运及组织学情况。WR2721预处理显著提高了7天存活率（91%对33%，P < 0.02）。在第4天，WR2721处理组的黏膜完整性和肠道净吸收均显著优于安慰剂组（P < 0.05）。因此，辐射诱导的回肠黏膜损伤伴随着体内回肠对水和电解质的净吸收减少。此外，WR2721预处理可提高动物存活率，同时减轻回肠损伤并改善肠道吸收。
