Hutton J C
Department of Clinical Biochemistry, Addenbrooke's Hospital, Cambridge, UK.
Diabetologia. 1994 Sep;37 Suppl 2:S48-56. doi: 10.1007/BF00400826.
The insulin storage granule of the pancreatic beta cell is assembled within the trans Golgi network from around 50 or so gene products many of which are synthesized coordinately with the major component, proinsulin. An important contribution to our understanding of the regulation of this process has come from studies of the post-translational processing of proinsulin and of other proteins which are stored in the granule, particularly the processing enzymes themselves. The present review focusses on recent insights into the molecular nature of the processing machinery, and the granule Ca(2+)-dependent subtilisin-related endopeptidases which catalyse the initial rate-limiting step in the enzymic conversion of proinsulin.
胰腺β细胞的胰岛素储存颗粒是在反式高尔基体网络中由大约50种左右的基因产物组装而成，其中许多基因产物与主要成分胰岛素原协同合成。对胰岛素原和其他储存于颗粒中的蛋白质（尤其是加工酶本身）进行的翻译后加工研究，为我们理解这一过程的调控做出了重要贡献。本综述重点关注加工机制的分子本质以及颗粒中依赖钙离子的枯草杆菌蛋白酶相关内肽酶的最新见解，这些酶催化胰岛素原酶促转化中的初始限速步骤。
