雄性蝾螈（Triturus carnifex Laur）繁殖过程中血浆催乳素浓度的季节性变化

Seasonal changes of plasma prolactin concentration in the reproduction of the crested newt (Triturus carnifex Laur).

作者信息

Mosconi G, Yamamoto K, Kikuyama S, Carnevali O, Mancuso A, Vellano C

机构信息

Department of Biology (MCA), University of Camerino, Italy.

出版信息

Gen Comp Endocrinol. 1994 Sep;95(3):342-9. doi: 10.1006/gcen.1994.1132.

DOI:10.1006/gcen.1994.1132

PMID:7821770

Abstract

Plasma prolactin (PRL) and vitellogenin (VTG) concentrations were determined during the annual reproductive cycle of the newt Triturus carnifex Laur. Plasma PRL was measured by a radioimmunoassay using purified newt (Cynops pyrrogaster) PRL as standard and radioligand and its antibodies. Using ELISA, plasma VTG titers were related to ovarian weight. The trends in PRL differed in the two sexes: the PRL changes seemed to be related with vitellogenic processes in the female, whereas they were more related to reproductive behavior in the male.

摘要

在火蝾螈（Triturus carnifex Laur.）的年度繁殖周期中测定了血浆催乳素（PRL）和卵黄蛋白原（VTG）浓度。血浆PRL采用放射免疫分析法测定，以纯化的蝾螈（Cynops pyrrogaster）PRL作为标准品、放射性配体及其抗体。使用酶联免疫吸附测定法（ELISA），血浆VTG滴度与卵巢重量相关。PRL的变化趋势在两性中有所不同：雌性的PRL变化似乎与卵黄生成过程有关，而雄性的PRL变化则更多地与生殖行为有关。