Horrocks L A, Toews A D, Thompson D K, Chin J Y
Adv Exp Med Biol. 1976;72:37-54. doi: 10.1007/978-1-4684-0955-0_5.
The important problem of membrane assembly and disassembly can be studied by measurements of rates of tunrover of labeled components. After intracerebral injections of ethanolamine and glycerol into mice, we have found rapid and slow turnover pools of 1,2-diacyl-sn-glycero-3-phosphorylethanolamine (diacyl-GPE) and 1,2-diacyl-sn-glycero-3-phosphorylcholine (diacyl-GPC). We have described the methods for calculation of half-lives for two or more pools and for the calculation of the relative size of the pools. For mice injected between 5 and 8 weeks of age, the rapid turnover pools have a half-life of 1.5 and 1.8 days for diacyl-GPC and diacyl-Ge respectively. Corresponding half-lives for the slow turnover pools are 20 and 27 days. The slow turnover pools include 74% of the diacyl-GPC and 86% of the diacyl-Ge. These turnover rates are in agreement with the flux of fatty acids through the diacylglycerol pools. We have proposed that the rapid turnover pool may be the phosphoglycerides that are exchangable with cytosol carrier proteins and that the slow turnover may represent the catabolism of membrane segments including the intrinsic proteins.
膜组装与拆卸的重要问题可通过测量标记成分的周转速率来研究。在向小鼠脑内注射乙醇胺和甘油后，我们发现了1,2 - 二酰基 - sn - 甘油 - 3 - 磷酸乙醇胺（二酰基 - GPE）和1,2 - 二酰基 - sn - 甘油 - 3 - 磷酸胆碱（二酰基 - GPC）的快速和慢速周转池。我们描述了计算两个或多个池的半衰期以及计算池相对大小的方法。对于在5至8周龄之间注射的小鼠，二酰基 - GPC和二酰基 - Ge的快速周转池半衰期分别为1.5天和1.8天。慢速周转池的相应半衰期为20天和27天。慢速周转池包含74%的二酰基 - GPC和86%的二酰基 - Ge。这些周转率与脂肪酸通过二酰基甘油池的通量一致。我们提出快速周转池可能是可与胞质溶胶载体蛋白交换的磷酸甘油酯，而慢速周转可能代表包括内在蛋白在内的膜片段的分解代谢。
