Wei Z G, Cotsarelis G, Sun T T, Lavker R M
Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia.
Invest Ophthalmol Vis Sci. 1995 Jan;36(1):236-46.
To determine the cell kinetic properties of epithelial cells from various zones of the conjunctiva.
The morphology and cell kinetics of bulbar, fornical, and palpebral conjunctival epithelium were studied in neonatal and adult SENCAR mice. To examine the proliferative rate of the conjunctival epithelium, a single administration of tritiated thymidine (3H-TdR) was used to detect cells in "S" phase. Proliferative rates were also assessed by determining mitotic activity after an intraperitoneal injection of colchicine to arrest cells in mitosis. To detect slow-cycling cells, mice received 3H-TdR continuously for 1 week. After a 4-week chase, animals were sacrificed and eyes were surgically removed. All tissues were immediately fixed in formalin and processed for histology and autoradiography.
Slow-cycling cells, detected as label-retaining cells (LRCs), were identified in bulbar, fornical, and palpebral epithelia, as well as in limbal epithelium. The greatest number of LRCs was found in fornical epithelium. In addition, we found a number of label-retaining goblet cells. This cell population was shown to incorporate 3H-TdR after a single pulse administration, and mitotic figures were seen in goblet cells after colchicine treatment, indicating that conjunctival goblet cells have proliferative capabilities.
These findings are consistent with earlier in vitro data that the fornical epithelium may be a zone enriched in conjunctival epithelial stem cells. This has important implications in conjunctival epithelial development and is relevant in wound repair. Furthermore, the concept that goblet cells are slow-cycling cells with proliferative capabilities provides new insights into the area of conjunctival homeostasis.
确定结膜不同区域上皮细胞的细胞动力学特性。
在新生和成年SENCAR小鼠中研究球结膜、穹窿结膜和睑结膜上皮的形态学和细胞动力学。为检测结膜上皮的增殖率，单次给予氚标记胸腺嘧啶核苷（3H-TdR）以检测处于“S”期的细胞。通过腹腔注射秋水仙碱使细胞停滞于有丝分裂期后测定有丝分裂活性，也可评估增殖率。为检测慢循环细胞（slow-cycling cells），小鼠连续1周接受3H-TdR。经过4周的追踪后，处死动物并手术摘除眼睛。所有组织立即用福尔马林固定，并进行组织学和放射自显影处理。
在球结膜、穹窿结膜和睑结膜上皮以及角膜缘上皮中鉴定出作为标记保留细胞（LRCs）的慢循环细胞。在穹窿结膜上皮中发现的LRCs数量最多。此外，我们发现了一些标记保留杯状细胞。在单次脉冲给药后，这群细胞显示摄取了3H-TdR，并且在秋水仙碱处理后的杯状细胞中可见有丝分裂图，表明结膜杯状细胞具有增殖能力。
这些发现与早期体外数据一致，即穹窿结膜上皮可能是结膜上皮干细胞富集的区域。这在结膜上皮发育中具有重要意义，并且与伤口修复相关。此外，杯状细胞是具有增殖能力的慢循环细胞这一概念为结膜稳态领域提供了新的见解。
