Drosinos E H, Board R G
University of Bath, School of Biology and Biochemistry, Claverton Down, UK.
J Appl Bacteriol. 1994 Dec;77(6):613-20. doi: 10.1111/j.1365-2672.1994.tb02809.x.
Pseudomonas fragi, Ps. lundensis and Ps. fluorescens were studied in axenic batch cultures growing in a lamb juice (pH 6.0) aerobically or in an atmosphere (Ps. fragi only) enriched with carbon dioxide at 4 degrees C. With all but a glucose dehydrogenase-deficient strain of Ps. fluorescens there was a sequential catabolism of glucose and lactate. Diauxic growth was observed in a nutrient-deficient meat juice supplemented with glucose and lactate. A transient peak in the concentration of gluconate and pyruvate was associated with the catabolism of glucose and lactate respectively. With Ps. fluorescens deficient in glucose dehydrogenase there was simultaneous catabolism of glucose and lactate. The stereoisomers of lactate were catabolized simultaneously in a laboratory medium. Glucose-6-phosphate was oxidized to 6-phosphogluconate by Ps. fragi and Ps. lundensis under aerobic conditions only. Creatine and creatinine were catabolized by Ps. fragi under aerobic conditions only. There was a slight decrease in the concentration of total amino acids (ninhydrin-reactive material) during the exponential phase of growth. The results suggest that the dominance of Ps. fragi in the climax populations in meat is due to catabolism of amino acid related substrates, creatine and creatinine.
在有氧条件下，以羊肉汁（pH 6.0）为培养基进行无菌分批培养，或在4℃、富含二氧化碳的气氛中（仅针对脆弱假单胞菌）对脆弱假单胞菌、伦德假单胞菌和荧光假单胞菌进行了研究。除了一株缺乏葡萄糖脱氢酶的荧光假单胞菌外，其他菌株对葡萄糖和乳酸都存在顺序分解代谢。在添加了葡萄糖和乳酸的营养缺乏型肉汁中观察到了二次生长现象。葡萄糖酸和丙酮酸浓度的瞬时峰值分别与葡萄糖和乳酸的分解代谢相关。对于缺乏葡萄糖脱氢酶的荧光假单胞菌，葡萄糖和乳酸同时进行分解代谢。在实验室培养基中，乳酸的立体异构体同时被分解代谢。仅在有氧条件下，脆弱假单胞菌和伦德假单胞菌可将6-磷酸葡萄糖氧化为6-磷酸葡萄糖酸。仅在有氧条件下，脆弱假单胞菌可分解肌酸和肌酐。在生长指数期，总氨基酸（茚三酮反应性物质）的浓度略有下降。结果表明，脆弱假单胞菌在肉类高峰期菌群中的优势地位归因于与氨基酸相关的底物、肌酸和肌酐的分解代谢。
