Hare G M, Evans P J, Mackinnon S E, Wade J A, Young A J, Hay J B
Trauma Research Program, Sunnybrook Health Science Centre, University of Toronto, North York, Ont., Canada.
J Neuroimmunol. 1995 Jan;56(1):9-16. doi: 10.1016/0165-5728(94)00120-d.
Cold-preservation of peripheral nerve allografts in vitro (3 weeks, 5 degrees C) was performed to determine its effect on local lymphocyte migration patterns in vivo. Lymphocyte migration was assessed by continuously monitoring the cell output in the regional lymph for nearly 1 month. Cold-preservation delayed or prevented the typical biphasic increase in efferent lymphocyte output observed after fresh allograft implantation. It also decreased the output of activated lymphocytes (CD 5 and MHC class II positive) compared with that seen in the fresh allograft response. These changes suggest that the host immune response to preserved nerve allografts is altered over a prolonged period in vivo (3 weeks). Cold-preservation may be a useful method of reducing allograft immunogenicity, thereby limiting systemic immunosuppression requirements for the successful clinical utilization of peripheral nerve allografts.
对周围神经同种异体移植物进行体外冷保存（3周，5摄氏度），以确定其对体内局部淋巴细胞迁移模式的影响。通过持续监测区域淋巴结中的细胞输出近1个月来评估淋巴细胞迁移。冷保存延迟或阻止了新鲜同种异体移植物植入后观察到的传出淋巴细胞输出典型的双相增加。与新鲜同种异体移植物反应相比，它还降低了活化淋巴细胞（CD5和MHC II类阳性）的输出。这些变化表明，宿主对保存的神经同种异体移植物的免疫反应在体内长期（3周）发生改变。冷保存可能是一种降低同种异体移植物免疫原性的有用方法，从而限制外周神经同种异体移植物成功临床应用所需的全身免疫抑制。
