Anderson C R, Edwards S L
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Australia.
J Neurosci Methods. 1994 Aug;53(2):137-41. doi: 10.1016/0165-0270(94)90170-8.
The ability of intraperitoneal injections of a retrograde neuronal tracer, Fluorogold, to label the entire population of sympathetic preganglionic neurones was tested with a double-labelling strategy. Animals were injected intraperitoneally (i.p.) with Fluorogold, while Fast Blue or subunit B of cholera toxin were injected into a peripheral autonomic ganglion or into the adrenal gland. Sympathetic preganglionic neurones were then examined for retrogradely transported tracers. In all cases, preganglionic neurones labelled with Fast Blue or cholera toxin also contained Fluorogold, indicating that i.p. injections of Fluorogold do reliably label the entire population of sympathetic preganglionic neurones.
采用双重标记策略，测试了经腹腔注射逆行神经元示踪剂荧光金标记整个交感神经节前神经元群体的能力。给动物腹腔内注射荧光金，同时将快蓝或霍乱毒素B亚单位注射到外周自主神经节或肾上腺中。然后检查交感神经节前神经元中逆行运输的示踪剂。在所有情况下，用快蓝或霍乱毒素标记的节前神经元也含有荧光金，这表明腹腔注射荧光金确实能可靠地标记整个交感神经节前神经元群体。
