垂体腺苷酸环化酶激活肽（PACAP）对大鼠肾上腺分泌活动的影响：初步体外研究。

Effects of pituitary adenylate-cyclase activating peptide (PACAP) on the rat adrenal secretory activity: preliminary in-vitro studies.

作者信息

Andreis P G, Malendowicz L K, Belloni A S, Nussdorfer G G

机构信息

Department of Anatomy, University of Padua, Italy.

出版信息

Life Sci. 1995;56(2):135-42. doi: 10.1016/0024-3205(94)00423-p.

DOI:10.1016/0024-3205(94)00423-p

PMID:7823760

Abstract

PACAP did not affect secretory activity of dispersed rat adrenocortical cells, but it markedly raised aldosterone (ALDO) and corticosterone (B) production by adrenal slices, containing both medullary and cortical tissues. The secretagogue effects of PACAP were suppressed by PACAP(6-38), a specific competitive antagonist. Isoprenaline (IP) enhanced ALDO, but not B secretion of adrenal slices, and l-alprenolol (AL) completely blocked IP effect. AL and corticotropin-inhibiting peptide (CIP) partially reversed ALDO response to a maximal effective concentration of PACAP; AL did not affect B response to a maximal effective concentration of PACAP, while CIP completely annulled it. Quarters of regenerated adrenocortical autotransplants, that are completely deprived of chromaffin cells, though displaying ALDO and B responses to IP and ACTH, were insensitive to PACAP. The hypothesis is advanced that adrenal medulla plays a pivotal role in the mechanism(s) underlying the adrenocortical secretagogue action of PACAP, being mineralocorticoid and glucocorticoid responses probably mediated by the release by chromaffin cells of catecholamine and ACTH or exclusively ACTH, respectively.

摘要

垂体腺苷酸环化酶激活肽（PACAP）不影响分散的大鼠肾上腺皮质细胞的分泌活性，但它能显著提高包含髓质和皮质组织的肾上腺切片中醛固酮（ALDO）和皮质酮（B）的生成。PACAP的促分泌作用被特异性竞争性拮抗剂PACAP(6 - 38)所抑制。异丙肾上腺素（IP）增强肾上腺切片中ALDO的分泌，但不影响B的分泌，而左旋普萘洛尔（AL）完全阻断IP的作用。AL和促肾上腺皮质激素抑制肽（CIP）部分逆转了肾上腺切片对最大有效浓度PACAP的ALDO反应；AL不影响肾上腺切片对最大有效浓度PACAP的B反应，而CIP则完全消除了该反应。完全去除嗜铬细胞的再生肾上腺皮质自体移植组织的四分之一，虽然对IP和促肾上腺皮质激素（ACTH）显示出ALDO和B反应，但对PACAP不敏感。由此提出一个假说：肾上腺髓质在PACAP对肾上腺皮质的促分泌作用机制中起关键作用，醛固酮和糖皮质激素反应可能分别由嗜铬细胞释放儿茶酚胺和ACTH或仅由ACTH介导。