整合于小鼠杂交瘤细胞染色体免疫球蛋白μ位点的重复Cμ序列之间的高频基因转换。
High-frequency gene conversion between repeated C mu sequences integrated at the chromosomal immunoglobulin mu locus in mouse hybridoma cells.
作者信息
Baker M D, Read L R
机构信息
Department of Veterinary Microbiology, University of Guelph, Ontario, Canada.
出版信息
Mol Cell Biol. 1995 Feb;15(2):766-71. doi: 10.1128/MCB.15.2.766.
Abstract
The occurrence of mitotic recombination between repeated immunoglobulin mu gene constant (C mu) region sequences stably integrated at the haploid chromosomal immunoglobulin mu locus in murine hybridoma cells was investigated. Recombination events are detected as changes in hapten-specific immunoglobulin M production. Recombination occurs with high frequency (0.5 to 0.8%) by a mechanism consistent with gene conversion. A double-strand break repair-like mechanism is suggested by the finding that repair of a 2-bp deletion mutation and a 2-bp insertion mutation occurs with parity in a donor-directed manner. The results also suggest that the gene conversion process is directional in that the 5' C mu region sequence is preferentially converted.
摘要
研究了在小鼠杂交瘤细胞单倍体染色体免疫球蛋白μ基因座上稳定整合的重复免疫球蛋白μ基因恒定（Cμ）区序列之间有丝分裂重组的发生情况。重组事件通过半抗原特异性免疫球蛋白M产生的变化来检测。重组以与基因转换一致的机制高频发生（0.5%至0.8%）。发现2个碱基对缺失突变和2个碱基对插入突变以供体定向方式同等程度地修复，这提示了一种类似双链断裂修复的机制。结果还表明基因转换过程是有方向性的，即5' Cμ区序列优先被转换。
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