Baker M D, Read L R
Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
Somat Cell Mol Genet. 1993 Jul;19(4):299-311. doi: 10.1007/BF01232743.
We have introduced a pSV2neo-derived vector that contains a 2-base-pair (bp) deletion in its immunoglobulin mu gene constant region into hybridoma cells bearing a single copy of the wild-type chromosomal immunoglobulin mu gene. Homologous recombination between the transferred mutant C mu region and the wild-type chromosomal C mu region is expected to introduce the 2-bp deletion into the chromosomal mu gene, generating recombinant cells synthesizing noncytolytic IgM. Analysis of the DNA in independent noncytolytic transformants indicates that in one case the mu gene has the structure expected for correct homologous recombination. Unexpectedly, the remaining transformants, bear chromosomal mu gene deletions.
我们已将一种源自pSV2neo的载体导入带有野生型染色体免疫球蛋白μ基因单拷贝的杂交瘤细胞，该载体在其免疫球蛋白μ基因恒定区存在一个2碱基对（bp）的缺失。预期转移的突变Cμ区域与野生型染色体Cμ区域之间的同源重组会将2-bp缺失引入染色体μ基因，从而产生合成非细胞溶解性IgM的重组细胞。对独立的非细胞溶解性转化体中的DNA分析表明，在一种情况下，μ基因具有正确同源重组预期的结构。出乎意料的是，其余的转化体带有染色体μ基因缺失。
