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脑微管相关蛋白MAP1A的纯化

Purification of brain microtubule-associated protein MAP1A.

作者信息

Pazzagli C, Avila J

机构信息

Centro de Biología Molecular, Severo Ochoa, (CSIC-UAM), Universidad Autónoma de Madrid, Spain.

出版信息

Neurochem Res. 1994 Sep;19(9):1195-8. doi: 10.1007/BF00965155.

DOI:10.1007/BF00965155
PMID:7824074
Abstract

Brain microtubule-associated protein MAP1A has been purified until homogeneity by using a novel procedure involving copolymerization with microtubules, treatment with poly-L-aspartic acid and FPLC. The purified protein retains its capacity to facilitate microtubule assembly.

摘要

脑微管相关蛋白MAP1A已通过一种新方法纯化至同质，该方法包括与微管共聚、用聚-L-天冬氨酸处理及快速蛋白质液相色谱法。纯化后的蛋白质保留了促进微管组装的能力。

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本文引用的文献

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Depletion of casein kinase II by antisense oligonucleotide prevents neuritogenesis in neuroblastoma cells.反义寡核苷酸使酪蛋白激酶II耗竭可阻止神经母细胞瘤细胞的神经突形成。
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Sulphonate buffers affect the recovery of microtubule-associated proteins MAP1 and MAP2: evidence that MAP1A promotes microtubule assembly.
磺酸盐缓冲液影响微管相关蛋白MAP1和MAP2的回收：MAP1A促进微管组装的证据。
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Microtubule-associated protein MAP1 promotes microtubule assembly in vitro.微管相关蛋白MAP1在体外促进微管组装。
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Heterogeneity of microtubule-associated protein 2 during rat brain development.大鼠脑发育过程中微管相关蛋白2的异质性
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Microtubule assembly in the absence of added nucleotides.在未添加核苷酸的情况下微管装配。
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Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules.猪神经微管体外组装与拆卸的比浊法研究。
J Mol Biol. 1974 Nov 15;89(4):737-55. doi: 10.1016/0022-2836(74)90048-5.
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Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.人红细胞膜主要多肽的电泳分析。
Biochemistry. 1971 Jun 22;10(13):2606-17. doi: 10.1021/bi00789a030.