Miura M, Okada J, Takayama K, Suzuki T
Department of Physiology 1st Division, Gunma University School of Medicine, Maebashi-shi, Japan.
Neurosci Lett. 1994 Sep 12;178(2):227-30. doi: 10.1016/0304-3940(94)90765-x.
After inhalation of 100% N2 (anoxia) or 8% CO2 (hypercapnia), neurons that express Fos protein (FOS) and/or Jun protein (JUN) were immunohistochemically identified in the CNS of rats anesthetized with urethane and alpha-chloralose. JUN-labeled cells were characteristically found in the respiration-related motor nuclei of the medulla and spinal cord, and in the central chemoreceptive area of the ventral medullary surface on which hypercapnia was more effective than anoxia, whereas FOS-labeled cells were dominant in the respiration-related sensory nuclei of the medulla on which anoxia was more effective than hypercapnia. The results suggest that the peripheral chemoreceptor and the central chemoreceptive pathways in the medulla and spinal cord may be traced by a combination of FOS and JUN expression methods.
在用乌拉坦和α-氯醛糖麻醉的大鼠中枢神经系统中，通过免疫组织化学方法鉴定了吸入100%氮气（缺氧）或8%二氧化碳（高碳酸血症）后表达Fos蛋白（FOS）和/或Jun蛋白（JUN）的神经元。JUN标记的细胞主要出现在延髓和脊髓与呼吸相关的运动核以及延髓腹侧表面的中枢化学感受区，在该区域高碳酸血症比缺氧更有效，而FOS标记的细胞在延髓与呼吸相关的感觉核中占主导地位，在该区域缺氧比高碳酸血症更有效。结果表明，通过FOS和JUN表达方法的联合应用，可以追踪外周化学感受器以及延髓和脊髓中的中枢化学感受通路。
