Chu W P, Liu W K, Ward D N
Biochim Biophys Acta. 1976 Jul 21;437(2):377-83. doi: 10.1016/0304-4165(76)90007-6.
The three tryptic glycopeptides from ovine lutropin, in which two were from the alpha-subunit (alpha-56 and alpha-82 glycopeptides) and one from the beta-subunits (beta-13 glycopeptide), have been isolated and their carohydrate compositions analyzed. The results indicate that the alpha-56 glycopeptide has the highest amount of carbohydrate, whereas the beta-13 glycopeptide has the least. In general, each of the glycopeptides has similar distribution of various sugars, i.e. high in mannose and glucosamine and low in fucose, sialic acid, galactose and galactosamine. Within the limit of experimental error, the sum of their carbohydrate composition is in agreement with the published data on the intact hormone or separated subunits.
已从羊促黄体激素中分离出三种胰蛋白酶消化的糖肽,其中两种来自α亚基(α-56和α-82糖肽),一种来自β亚基(β-13糖肽),并对其碳水化合物组成进行了分析。结果表明,α-56糖肽的碳水化合物含量最高,而β-13糖肽的含量最少。一般来说,每种糖肽的各种糖类分布相似,即甘露糖和氨基葡萄糖含量高,岩藻糖、唾液酸、半乳糖和半乳糖胺含量低。在实验误差范围内,它们碳水化合物组成的总和与已发表的关于完整激素或分离亚基的数据一致。