Schneider E G, Kennedy E P
Biochim Biophys Acta. 1976 Aug 23;441(2):294-301. doi: 10.1016/0005-2760(76)90172-7.
The liponucleotide fraction of Saccharomyces cerevisiae was isolated from cells grown on tritiated uracil and identified as CDPdiacylglycerol on the basis of (1), its behavior as a single compound upon DEAE-cellulose and thin-layer chromatography; (2), its extreme lability to mild alkaline methanolysis; and (3), its hydrolysis by the CDPdiacylglycerol hydrolase of Escherichia coli to yield tritiated 5'-CMP. At most, only about 5% of yeast liponucleotide could be dCDPdiacylglycerol, in contrast to the presence of nearly equimolar amounts of CDP-and dCDPdiacylglycerols in E. coli. Although no CDPceramide could be detected in the liponucleotide fraction of this organism, the possibility still exists that it may be an intermediate in the biosynthesis of sphingolipids in systems yet to be examined.
酿酒酵母的脂核苷酸部分是从在氚标记尿嘧啶上生长的细胞中分离出来的，并根据以下几点被鉴定为CDP二酰甘油：（1）它在DEAE - 纤维素和薄层层析上作为单一化合物的行为；（2）它对温和碱性甲醇解的极端不稳定性；（3）它被大肠杆菌的CDP二酰甘油水解酶水解产生氚标记的5'-CMP。与大肠杆菌中存在几乎等摩尔量的CDP - 和dCDP二酰甘油相反，酵母脂核苷酸中最多只有约5%可能是dCDP二酰甘油。尽管在该生物体的脂核苷酸部分中未检测到CDP神经酰胺，但在尚未研究的系统中，它仍有可能是鞘脂生物合成的中间体。
