Uehara Y, Hori M, Umezawa H
Biochim Biophys Acta. 1976 Aug 18;442(2):251-62. doi: 10.1016/0005-2787(76)90495-0.
The effect of negamycin and its derivatives on protein synthesis in an Escherichia coli cell-free system was determined. (1) Unlike tetracycline and streptomycin, negamycin did not inhibit codon-specific binding of aminoacyl-tRNA to ribosomes. (2) The miscoding activity of the genamycin family compounds was not parallel with the activity inhibiting the termination reaction. (3) Although negamycin strongly inhibited the overall termination reaction, it inhibited only slightly the two substeps involved; the formation of releasing factor 1 - U-A-[3H]G - ribosome complex and the peptidyl-transferase action (release of formylmethionine from the initiation complex by puromycin). (4) The termination reaction performed with ribosomes from either streptomycin- or kanamycin-resistant E. coli cells was sensitive to negamycin. These results indicate that the inhbitory effect of negamycin on the termination reaction is specific to negamycin and distinct from that of tetracycline and streptomycin.
测定了Negamycin及其衍生物对大肠杆菌无细胞体系中蛋白质合成的影响。（1）与四环素和链霉素不同，Negamycin不抑制氨酰-tRNA与核糖体的密码子特异性结合。（2）庆大霉素家族化合物的错义编码活性与抑制终止反应的活性不平行。（3）虽然Negamycin强烈抑制整体终止反应，但仅轻微抑制所涉及的两个子步骤；释放因子1-U-A-[3H]G-核糖体复合物的形成和肽基转移酶作用（嘌呤霉素从起始复合物中释放甲酰甲硫氨酸）。（4）用来自抗链霉素或抗卡那霉素的大肠杆菌细胞的核糖体进行的终止反应对Negamycin敏感。这些结果表明，Negamycin对终止反应的抑制作用是Negamycin特有的，且与四环素和链霉素不同。
