Sollars S I, Bernstein I L
Department of Psychology, University of Washington.
Behav Neurosci. 1994 Oct;108(5):981-7. doi: 10.1037//0735-7044.108.5.981.
Amiloride-sensitive sodium channels in taste buds appear to play a key role in the response to NaCl stimulation, at least in adult rats. The researchers examined whether neonatal rats, which display an exaggerated preference for hypertonic NaCl solutions, lack functional amiloride-sensitive sodium channels. NaCl intake was significantly reduced by amiloride pretreatment, but water and ammonium chloride, NH4Cl, were unaffected. The researchers assessed whether the early appearance of amiloride sensitivity was mediated by effects on chorda tympani (CT) activity by sectioning the CT before testing. CT transection reduced intake of NaCl solutions and eliminated evidence of amiloride sensitivity. Amiloride sensitivity was also assessed by recording of whole-nerve CT activity at 8-11 days of age; the response to NaCl stimulation was significantly suppressed by amiloride. These data indicate that amiloride-sensitive sodium channels develop earlier than previously believed.
味蕾中的氨氯地平敏感钠通道似乎在对氯化钠刺激的反应中起关键作用，至少在成年大鼠中是这样。研究人员研究了对高渗氯化钠溶液表现出过度偏好的新生大鼠是否缺乏功能性氨氯地平敏感钠通道。氨氯地平预处理显著降低了氯化钠的摄入量，但水和氯化铵（NH4Cl）不受影响。研究人员通过在测试前切断鼓索神经（CT）来评估氨氯地平敏感性的早期出现是否由对鼓索神经活动的影响介导。切断鼓索神经减少了氯化钠溶液的摄入量，并消除了氨氯地平敏感性的证据。还通过记录8至11日龄全神经鼓索神经活动来评估氨氯地平敏感性；氨氯地平显著抑制了对氯化钠刺激的反应。这些数据表明，氨氯地平敏感钠通道的发育比以前认为的要早。
