McKenzie D, Henderson A R
Clin Chim Acta. 1976 Jul 15;70(2):333-6. doi: 10.1016/0009-8981(76)90436-8.
In patients with end-stage renal disease requiring maintenance haemodialysis we have observed that serum albumin becomes fluorescent (excitation 356 nm, emission above 405 nm). This fluorescence is detected in the fluorescent assay of lactate dehydrogenase (LDH) isoenzymes on thin-layer agarose creating the impression of an additional LDH isoenzyme anodal to LDH1. This artefact disappears, or is much reduced, following successful donor kidney transplantation. It is not seen in any other pathological condition. This phenomenon can readily be detected by means of "blank" assays on the agarose plates after specimen application and after electrophoresis of the specimen without the substrate overlay.
在需要维持性血液透析的终末期肾病患者中，我们观察到血清白蛋白会产生荧光（激发波长356nm，发射波长高于405nm）。在薄层琼脂糖上进行乳酸脱氢酶（LDH）同工酶的荧光测定时可检测到这种荧光，造成在LDH1阳极出现一种额外LDH同工酶的假象。成功进行供体肾移植后，这种假象消失或大大减轻。在任何其他病理状况下均未见到这种现象。在加样后以及标本在不覆盖底物的情况下进行电泳后，通过琼脂糖平板上的“空白”测定可轻易检测到这种现象。
