Lebel L, Gabrielsson J, Laurent T C, Gerdin B
Department of Biomedical Research, Pharmacia AB, Uppsala, Sweden.
Eur J Clin Invest. 1994 Sep;24(9):621-6. doi: 10.1111/j.1365-2362.1994.tb01114.x.
The elimination of intravenously injected hyaluronan (HA) from the blood was investigated in 12 healthy volunteers. Three consecutive 30 min infusions of HA were given, separated by 90 min washout periods. Blood samples were taken before, during and after each infusion and the plasma HA concentration was determined. The deposition of HA was modelled according to a Michaelis-Menten kinetic model which included natural synthesis of HA. Km and Vmax was estimated to 0.34 +/- 0.13 microgram ml-1 and 3.48 +/- 0.97 microgram min-1 kg-1 b.w., respectively. The endogenous input was calculated to be 24 +/- 11 micrograms min-1 and was found to correlate to the age of the subjects (P < 0.05). As the baseline HA concentration was 0.031 +/- 0.21 microgram ml-1, the rate of elimination was linear in the normal concentration range. The calculated Vd was about 75% higher than a weight-estimated plasma volume. The total amount of HA excreted by the kidneys during the study period was 394 +/- 77 micrograms, which corresponded to approximately 1.7% of the total input of HA into the circulation during the experiment.
在12名健康志愿者中研究了静脉注射透明质酸（HA）从血液中的消除情况。连续进行三次30分钟的HA输注，每次输注之间间隔90分钟的洗脱期。在每次输注前、输注期间和输注后采集血样，并测定血浆HA浓度。根据包含HA自然合成的米氏动力学模型对HA的沉积进行建模。Km和Vmax估计分别为0.34±0.13微克/毫升和3.48±0.97微克/分钟/千克体重。内源性输入计算为24±11微克/分钟，并且发现与受试者的年龄相关（P<0.05）。由于基线HA浓度为0.031±0.21微克/毫升，在正常浓度范围内消除速率呈线性。计算得到的Vd比根据体重估算的血浆体积高约75%。在研究期间，肾脏排泄的HA总量为394±77微克，这大约相当于实验期间HA进入循环的总输入量的1.7%。
