Jensen N, Linblad B, Leide S, Bergqvist D
Department of Surgery, Lund University, Malmö General Hospital, Sweden.
Eur J Vasc Surg. 1994 Nov;8(6):690-3. doi: 10.1016/s0950-821x(05)80648-6.
To assess, in vivo, the loss of endothelial cells seeded in a Dacron graft at low, restricted arterial flow and at normal unrestricted flow.
Laboratory animal study.
Indium-111-oxine labelled endothelial cells were seeded in externally supported, preclotted Dacron grafts, inserted as interposition grafts in the carotid arteries of nine sheep. Activity (radioactivity) was measured, flow established, on one side unrestricted 120-180 ml/minute, and on the other restricted to 50 ml/minute by a distal clamp.
The reduction in activity over the grafts was measured for 2 hours.
There was an immediate loss of activity to around 80% and thereafter a lower rate of cell loss to around 50%. No difference could be demonstrated in the loss from low flow compared to high flow grafts. All grafts were patent. There was no difference in thrombus weights.
No difference could be demonstrated in seeded endothelial cell loss from a Dacron graft in vivo at low flow (50 ml/minute) compared with unrestricted flow (120-180 ml/minute).
在体内评估种植于涤纶移植物中的内皮细胞在低流量、受限动脉血流以及正常非受限血流情况下的损失情况。
实验动物研究。
将铟 - 111 - 氧嗪标记的内皮细胞种植于外部支撑的预凝血涤纶移植物中，作为插入移植物植入9只绵羊的颈动脉。测量活性（放射性），建立血流，一侧血流不受限，为120 - 180毫升/分钟，另一侧通过远端夹钳将血流限制在50毫升/分钟。
测量移植物上活性降低情况，持续2小时。
活性立即损失至约80%，此后细胞损失速率降低至约50%。与高流量移植物相比，低流量移植物的细胞损失无差异。所有移植物均保持通畅。血栓重量无差异。
与非受限血流（120 - 180毫升/分钟）相比，涤纶移植物在体内低流量（50毫升/分钟）情况下种植的内皮细胞损失无差异。
