Bellón J M, Buján J, Hernando A, Honduvilla N G, Jurado F
Department of Morphological Sciences and Surgery, (Surgical Research Laboratory), Faculty of Medicine, University of Alcalá de Henares, Madrid, Spain.
Eur J Vasc Surg. 1994 Nov;8(6):694-702. doi: 10.1016/s0950-821x(05)80649-8.
A comparative study has been carried out dealing with the vascular healing process in two experimental vascular graft models to determine the differences or similarities between the two. One of the models consisted of the use of arterial autografts and the other of the implantation of vascular microprostheses of polytetrafluoroethylene (PTFE). The common iliac artery of female Sprague-Dawley rats was used. The length of the grafts in both models was 5 mm. A microsurgical technique was employed, and anticoagulant and antibiotic therapies were not used. The results were studied using light microscopy, transmission and scanning electron microscopies, autoradiography, and immunohistochemistry. A patency of 100% was obtained in the arterial autografts, and 87.6% in the PTFE implants. The histopathological findings were as follows: a) the mechanisms of immediate postgrafting response were similar, with marked presence of fibrin and platelet deposition in the form of a nonthrombogenic monolayer; b) a "neoadventitia" formed over the implant in both models; c) the endothelialisation was complete in both types of grafts 2 to 3 weeks after implantation; d) an intimal hyperplastic response appeared in both, although at different times (in the first week in the autografts and at one month with the PTFE); e) white cell accumulation was significantly greater on the PTFE luminal surface than on the autograft. The intimal hyperplasia was formed mainly by secretory myocytes in the autografts, while in the PTFE implants, fibrosis predominated.
在两种实验性血管移植模型中开展了一项比较研究，以确定两者之间的差异或相似之处。其中一种模型使用动脉自体移植物，另一种模型植入聚四氟乙烯（PTFE）血管微型假体。选用雌性斯普拉格-道利大鼠的髂总动脉。两种模型中移植物的长度均为5毫米。采用显微外科技术，未使用抗凝和抗生素治疗。使用光学显微镜、透射和扫描电子显微镜、放射自显影术和免疫组织化学对结果进行研究。动脉自体移植物的通畅率为100%，PTFE植入物的通畅率为87.6%。组织病理学结果如下：a）移植后即刻反应机制相似，以非血栓形成单层形式存在大量纤维蛋白和血小板沉积；b）两种模型的植入物上均形成了“新外膜”；c）两种类型的移植物在植入后2至3周内皮化均完成；d）两者均出现内膜增生反应，尽管时间不同（自体移植物在第一周出现，PTFE在一个月时出现）；e）PTFE管腔表面的白细胞积聚明显多于自体移植物。自体移植物内膜增生主要由分泌性肌细胞形成，而在PTFE植入物中，纤维化占主导。
