Ono T, Suzuki T, Anraku Y, Iida H
Division of Cell Proliferation, National Institute for Basic Biology, Okazaki, Japan.
Gene. 1994 Dec 30;151(1-2):203-8. doi: 10.1016/0378-1119(94)90657-2.
The MID2 gene whose defect (the mid2-1 mutation) results in mating-pheromone-induced death in Saccharomyces cerevisiae was cloned and its nucleotide (nt) sequence determined. The sequence showed an open reading frame (ORF) coding for a 376-amino-acid (aa) protein with an estimated M(r) of 39,104, and six potential TATA boxes and two pheromone-response elements in its 5'-upstream region. The deduced aa sequence showed that the MID2 product (Mid2p) contains a putative N-terminal signal sequence followed by a long Ser-rich region that could contain O-glycosylation sites, a potential transmembrane domain and a conserved Ca(2+)-binding domain, with the latter two located in the C-terminal half. Northern blot analysis showed that the expression of MID2 is stimulated threefold by mating pheromone. Cells that lack MID2 were able to grow normally, but died when exposed to mating pheromone, like the original mid2-1 mutant.
克隆了酿酒酵母中缺陷（mid2 - 1突变）导致交配信息素诱导死亡的MID2基因，并确定了其核苷酸（nt）序列。该序列显示一个开放阅读框（ORF），编码一个376个氨基酸（aa）的蛋白质，估计分子量（M(r)）为39,104，并且在其5'上游区域有六个潜在的TATA框和两个信息素反应元件。推导的氨基酸序列表明，MID2产物（Mid2p）包含一个推定的N端信号序列，其后是一个可能包含O - 糖基化位点的长的富含丝氨酸区域、一个潜在的跨膜结构域和一个保守的Ca(2+)结合结构域，后两者位于C端的后半部分。Northern印迹分析表明，交配信息素可使MID2的表达提高三倍。缺乏MID2的细胞能够正常生长，但像原始的mid2 - 1突变体一样，在暴露于交配信息素时死亡。
