VIBT EQ Extremophile Center, Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria.
Genes (Basel). 2019 May 20;10(5):381. doi: 10.3390/genes10050381.
Salt mines are among the most extreme environments as they combine darkness, low nutrient availability, and hypersaline conditions. Based on comparative genomics and transcriptomics, we describe in this work the adaptive strategies of the true halophilic fungus , found in a salt mine in Austria, and compare this strain to the ex-type halotolerant fungal strain . On a genomic level, exhibits a reduced genome size compared to , as well as a contraction of genes involved in transport processes. The proteome of exhibits an increased proportion of alanine, glycine, and proline compared to the proteome of non-halophilic species. Transcriptome analyses of both strains growing at 5% and 20% NaCl show that regulates three-times fewer genes than in order to adapt to the higher salt concentration. In , the increased osmotic stress impacted processes related to translation, transcription, transport, and energy. In contrast, membrane-related and lignolytic proteins were significantly affected in .
盐矿是最极端的环境之一，因为它们结合了黑暗、低营养可用性和高盐条件。基于比较基因组学和转录组学，我们在这项工作中描述了在奥地利盐矿中发现的真正嗜盐真菌的适应策略，并将该菌株与外类群耐盐真菌菌株进行了比较。在基因组水平上，与外类群耐盐真菌菌株相比，表现出较小的基因组大小，以及与运输过程相关的基因收缩。与非嗜盐物种的蛋白质组相比，的蛋白质组中丙氨酸、甘氨酸和脯氨酸的比例增加。在 5%和 20%NaCl 下生长的两种菌株的转录组分析表明，为了适应更高的盐浓度，的基因调控是外类群耐盐真菌菌株的三倍。在外类群耐盐真菌菌株中，增加的渗透压应激影响了与翻译、转录、运输和能量相关的过程。相比之下，在中，与膜相关和木质素降解蛋白受到显著影响。
