Rodríguez-Maseda H, Musto H
Laboratoire de Génétique Moléculaire, Institut Jacques Monod, Paris, France.
Gene. 1994 Dec 30;151(1-2):221-4. doi: 10.1016/0378-1119(94)90660-2.
Fractionation of DNA from Trypanosoma brucei and T. equiperdum by centrifugation in a Cs2SO4/BAMD density gradient indicated that these genomes are compositionally compartmentalized, a conclusion confirmed by the analysis of the compositional distribution of third codon positions from T. brucei and T. cruzi. In order to investigate whether this compartmentalization is accompanied by the often different properties of coding sequences, we have analyzed and compared the compositional compartments with respect to dinucleotide frequency and amino-acid usage of the encoded proteins of all gene sequences available in the GenBank database from T. brucei and T. cruzi. In all cases, the compartments displayed remarkable differences. These results are similar to findings obtained in highly compartmentalized genomes, like those of warm-blooded vertebrates.
通过在Cs2SO4/BAMD密度梯度中离心对布氏锥虫和马媾疫锥虫的DNA进行分级分离，结果表明这些基因组在组成上是分区的，这一结论通过对布氏锥虫和克氏锥虫第三密码子位置的组成分布分析得到证实。为了研究这种分区是否伴随着编码序列通常不同的特性，我们分析并比较了GenBank数据库中布氏锥虫和克氏锥虫所有可用基因序列的二核苷酸频率和编码蛋白质的氨基酸使用情况方面的组成分区。在所有情况下，这些分区都表现出显著差异。这些结果与在高度分区的基因组（如温血脊椎动物的基因组）中获得的发现相似。
