Isacchi A, Bernardi G, Bernardi G
Laboratoire de Génétique Moléculaire, Institut Jacques Monod, Paris, France.
FEBS Lett. 1993 Dec 6;335(2):181-3. doi: 10.1016/0014-5793(93)80725-a.
High molecular weight DNA preparations from Trypanosoma brucei and Trypanosoma equiperdum were fractionated by preparative centrifugation in a Cs2SO4 density gradient in the presence of BAMD, bis(acetatomercurimethyl)dioxane, a sequence-specific DNA ligand. Analytical centrifugation in CsCl of the DNA fractions so obtained showed that both DNAs had a bimodal distribution with two major peaks banding at 1.702-1.703 and 1.708 g/cm3 and representing 1/3 and 2/3 of total DNA, respectively. Several minor components were also detected. These results indicate that a compositional compartmentalization is not only found in the genome of vertebrates and plants, as already described, but also in those of protozoa such as Trypanosomes.
来自布氏锥虫和马媾疫锥虫的高分子量DNA制剂，在序列特异性DNA配体双（乙酰汞甲基）二氧六环（BAMD）存在的情况下，通过在Cs2SO4密度梯度中进行制备性离心进行分级分离。对如此获得的DNA级分进行CsCl分析离心，结果表明两种DNA均具有双峰分布，两个主要峰分别在1.702 - 1.703和1.708 g/cm3处形成条带，分别占总DNA的1/3和2/3。还检测到几个次要成分。这些结果表明，如前所述，不仅在脊椎动物和植物的基因组中发现了组成分区，在原生动物如锥虫的基因组中也存在。
