Su X Z, Wellems T E
Laboratory of Malaria Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Gene. 1994 Dec 30;151(1-2):225-30. doi: 10.1016/0378-1119(94)90661-0.
A gene (pfhsp86) encoding a member of the heat-shock protein 90 (HSP90) family has been isolated from Plasmodium falciparum (Pf). The pfhsp86 coding region comprises two exons separated by an 0.8-kb intron with consensus splice junction sequences. The transcript itself is 3.4-kb long and includes a 0.65-kb 5'-untranslated region (UTR) and a 0.54-kb 3'-UTR. Upstream of the transcription start point (tsp) are putative promoter modules: an inverted CCAAT box, a G + C-rich sequence and several TATA sequences. Transcription is enhanced in in erythrocyte-stage parasites cultivated at elevated temperatures (2-3-fold at 39 degrees C and 3-4-fold at 41 degrees C). The pfhsp86 gene maps within a chromosome 7 segment that is linked to chloroquine (Cq) response in a Pf cross. The parents of this cross (Dd2, HB3) differ in the first exon by two trinucleotide repeats, while more divergence is apparent between the introns. These trinucleotide repeat differences are linked to Cq response in the HB3 x Dd2 cross, but they did not predict Cq response in nine Pf lines from different locations.
已从恶性疟原虫（Pf）中分离出一个编码热休克蛋白90（HSP90）家族成员的基因（pfhsp86）。pfhsp86编码区由两个外显子组成，中间隔着一个0.8 kb的内含子，内含子具有共有剪接连接序列。转录本本身长3.4 kb，包括一个0.65 kb的5'非翻译区（UTR）和一个0.54 kb的3'UTR。转录起始点（tsp）上游是假定的启动子模块：一个反向CCAAT框、一个富含G + C的序列和几个TATA序列。在高温（39℃时提高2 - 3倍，41℃时提高3 - 4倍）培养的红细胞期疟原虫中，转录增强。pfhsp86基因定位于7号染色体的一个片段内，该片段在Pf杂交中与氯喹（Cq）反应相关。该杂交的亲本（Dd2、HB3）在第一个外显子中有两个三核苷酸重复的差异，而内含子之间的差异更明显。这些三核苷酸重复差异与HB3×Dd2杂交中的Cq反应相关，但它们不能预测来自不同地点的9个Pf品系中的Cq反应。
