Reduced extension temperatures required for PCR amplification of extremely A+T-rich DNA.
作者信息
Su X Z, Wu Y, Sifri C D, Wellems T E
机构信息
Laboratory of Parasitic Diseases, National Institute of Allery and Infectious Disease, National Institutes of Health, Bethesda, MD 20892-0425, USA.
出版信息
Nucleic Acids Res. 1996 Apr 15;24(8):1574-5. doi: 10.1093/nar/24.8.1574.
Abstract
摘要
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Towards a high-resolution map of the Plasmodium falciparum genome.迈向恶性疟原虫基因组的高分辨率图谱。
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Long PCR.长片段聚合酶链反应
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Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction.双链核酸的热变性：对梯度凝胶电泳和聚合酶链反应关键温度的预测
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Sequence, transcript characterization and polymorphisms of a Plasmodium falciparum gene belonging to the heat-shock protein (HSP) 90 family.恶性疟原虫热休克蛋白（HSP）90家族一个基因的序列、转录本特征及多态性
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Use of vectorette and subvectorette PCR to isolate transgene flanking DNA.使用载体连接片段和亚载体连接片段PCR分离转基因侧翼DNA。
PCR Methods Appl. 1994 Oct;4(2):71-5. doi: 10.1101/gr.4.2.71.
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The genome of Plasmodium falciparum. I: DNA base composition.恶性疟原虫的基因组。I：DNA碱基组成。
Nucleic Acids Res. 1982 Jan 22;10(2):539-46. doi: 10.1093/nar/10.2.539.
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Recursion relation generation of probability profiles for specific-sequence macromolecules with long-range correlations.具有长程相关性的特定序列大分子概率分布的递归关系生成
Biopolymers. 1974;13(9):1859-71. doi: 10.1002/bip.1974.360130916.
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Codon usage in Plasmodium falciparum.恶性疟原虫的密码子使用情况。
Mol Biochem Parasitol. 1988 Jan 1;27(1):35-42. doi: 10.1016/0166-6851(88)90022-9.
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