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Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length.使用SYBR Green进行拷贝数估计：富含AT的DNA及扩增子长度变异所造成的干扰
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Low guanine content and biased nucleotide distribution in vertebrate mtDNA can cause overestimation of non-CpG methylation.脊椎动物线粒体DNA中鸟嘌呤含量低和核苷酸分布偏向性会导致非CpG甲基化的高估。
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Molecular typing of Cyclospora cayetanensis in produce and clinical samples using targeted enrichment of complete mitochondrial genomes and next-generation sequencing.采用靶向完整线粒体基因组富集和下一代测序技术对农产品和临床样本中的环孢子虫进行分子分型。
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本文引用的文献

1
Towards a high-resolution map of the Plasmodium falciparum genome.迈向恶性疟原虫基因组的高分辨率图谱。
Parasitol Today. 1992 Jul;8(7):225-9. doi: 10.1016/0169-4758(92)90118-l.
2
Long PCR.长片段聚合酶链反应
Nature. 1994 Jun 23;369(6482):684-5. doi: 10.1038/369684a0.
3
Effective amplification of long targets from cloned inserts and human genomic DNA.从克隆插入片段和人类基因组DNA中有效扩增长片段靶标。
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5695-9. doi: 10.1073/pnas.91.12.5695.
4
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.从λ噬菌体模板中以高保真度和高产量进行高达35千碱基DNA的PCR扩增。
Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2216-20. doi: 10.1073/pnas.91.6.2216.
5
Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction.双链核酸的热变性：对梯度凝胶电泳和聚合酶链反应关键温度的预测
Nucleic Acids Res. 1994 Jul 25;22(14):2760-8. doi: 10.1093/nar/22.14.2760.
6
Sequence, transcript characterization and polymorphisms of a Plasmodium falciparum gene belonging to the heat-shock protein (HSP) 90 family.恶性疟原虫热休克蛋白（HSP）90家族一个基因的序列、转录本特征及多态性
Gene. 1994 Dec 30;151(1-2):225-30. doi: 10.1016/0378-1119(94)90661-0.
7
Use of vectorette and subvectorette PCR to isolate transgene flanking DNA.使用载体连接片段和亚载体连接片段PCR分离转基因侧翼DNA。
PCR Methods Appl. 1994 Oct;4(2):71-5. doi: 10.1101/gr.4.2.71.
8
The genome of Plasmodium falciparum. I: DNA base composition.恶性疟原虫的基因组。I：DNA碱基组成。
Nucleic Acids Res. 1982 Jan 22;10(2):539-46. doi: 10.1093/nar/10.2.539.
9
Recursion relation generation of probability profiles for specific-sequence macromolecules with long-range correlations.具有长程相关性的特定序列大分子概率分布的递归关系生成
Biopolymers. 1974;13(9):1859-71. doi: 10.1002/bip.1974.360130916.
10
Codon usage in Plasmodium falciparum.恶性疟原虫的密码子使用情况。
Mol Biochem Parasitol. 1988 Jan 1;27(1):35-42. doi: 10.1016/0166-6851(88)90022-9.

Reduced extension temperatures required for PCR amplification of extremely A+T-rich DNA.

作者信息

Su X Z, Wu Y, Sifri C D, Wellems T E

机构信息

Laboratory of Parasitic Diseases, National Institute of Allery and Infectious Disease, National Institutes of Health, Bethesda, MD 20892-0425, USA.

出版信息

Nucleic Acids Res. 1996 Apr 15;24(8):1574-5. doi: 10.1093/nar/24.8.1574.

DOI:10.1093/nar/24.8.1574
PMID:8628694
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC145803/
Abstract
摘要