大鼠Gadd45 cDNA的克隆及其在脑中的mRNA表达
Cloning of the rat Gadd45 cDNA and its mRNA expression in the brain.
作者信息
Yoshida T, Schneider E L, Mori N
机构信息
Department of Biological Sciences, University of Southern California, Los Angeles 90089.
出版信息
Gene. 1994 Dec 30;151(1-2):253-5. doi: 10.1016/0378-1119(94)90666-1.
We cloned the rat Gadd45 (growth arrest and DNA damage inducible) cDNA and examined its mRNA induction by gamma-ray irradiation in the rat brain. The rat Gadd45 cDNA sequence was highly homologous to the previously published human and hamster cDNAs, and was partially similar to the 28S rRNA gene. The mRNA encoding rat GADD45 was induced in the brain after gamma-ray irradiation. This finding indicates that Gadd45 is an inducible gene following the ionizing radiation, not only in cultured cells in vitro, but also in animal tissues in vivo.
我们克隆了大鼠Gadd45（生长停滞和DNA损伤诱导基因）的cDNA，并检测了γ射线照射后其在大鼠脑中mRNA的诱导情况。大鼠Gadd45 cDNA序列与先前发表的人类和仓鼠cDNA高度同源，且与28S rRNA基因部分相似。编码大鼠GADD45的mRNA在γ射线照射后在脑中被诱导。这一发现表明，Gadd45是电离辐射后的一个诱导基因，不仅在体外培养细胞中如此，在体内动物组织中也是如此。
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