大鼠GADD45基因的克隆及体内电离辐射后的诱导分析。

Cloning of rat GADD45 gene and induction analysis following ionizing radiation in vivo.

作者信息

Yoshida T, Okazaki T, Hughes P E, Schneider E L, Mori N

机构信息

Division of Neurogerontology, University of Southern California, Los Angeles 90089, USA.

出版信息

FEBS Lett. 1996 Feb 12;380(1-2):87-92. doi: 10.1016/0014-5793(96)00012-9.

DOI:10.1016/0014-5793(96)00012-9

PMID:8603754

Abstract

A gene encoded GADD45 was isolated from rat and revealed four exons along with a p53 binding consensus sequence and a putative AP-1 site in the third intron. This suggests that the rat GADD45 gene is also involved in the p53 signal pathway related to the G1 cell cycle checkpoint. The rat GADD45 mRNA was induced within 30 min in liver and increased as a function of gamma-irradiation. We found that mRNA expression differed substantially in a variety of tissues (brain, liver, kidney, and spleen). The finding of in vivo induction of GADD45 gene may provide insight into the role of GADD45 gene in DNA repair.

摘要

从大鼠中分离出一种由基因编码的GADD45，它有四个外显子，在第三个内含子中有一个p53结合共有序列和一个推定的AP-1位点。这表明大鼠GADD45基因也参与了与G1细胞周期检查点相关的p53信号通路。大鼠GADD45 mRNA在肝脏中30分钟内被诱导，并随着γ射线照射而增加。我们发现，mRNA表达在多种组织（脑、肝、肾和脾）中存在显著差异。GADD45基因在体内被诱导这一发现可能有助于深入了解GADD45基因在DNA修复中的作用。