Heine D, Khambata S, Wydner K S, Passmore H C
Nelson Biological Laboratories, Rutgers University, Piscataway, New Jersey 08855-1059.
Genomics. 1994 Sep 1;23(1):168-77. doi: 10.1006/geno.1994.1474.
Most of the recombination events detected within the major histocompatibility complex (MHC) of the mouse fall into areas of limited physical size that have been designated recombinational hot spots. One of these hot spots, associated with the Ea gene, appears to be active only in the presence of the p haplotype of the MHC. To study the regulation of the Ea recombinational hot spot and its haplotype specificity, a high-resolution comparative map of the MHC and adjacent regions was completed in four different backcrosses carrying the p haplotype. This mapping study utilized a total of 29 PCR-based molecular markers, including 7 newly developed markers spanning the region between Pim1 and D17Mit11 on Chromosome 17. The analysis of a total of 1093 backcross animals: (1) revealed that the presence of the p haplotype of the MHC is not sufficient to induce recombination at the Ea hot spot in a dominant manner, and (2) resulted in the definition of a new intra-MHC recombinational hot spot between the Tnfb and the H2-D genes.
在小鼠主要组织相容性复合体（MHC）中检测到的大多数重组事件都发生在物理尺寸有限的区域，这些区域被指定为重组热点。其中一个与Ea基因相关的热点似乎仅在MHC的p单倍型存在时才活跃。为了研究Ea重组热点的调控及其单倍型特异性，在携带p单倍型的四个不同回交中完成了MHC及其相邻区域的高分辨率比较图谱。这项定位研究总共使用了29个基于PCR的分子标记，包括7个新开发的标记，跨越17号染色体上Pim1和D17Mit11之间的区域。对总共1093只回交动物的分析：（1）表明MHC的p单倍型的存在不足以以显性方式诱导Ea热点处的重组，并且（2）导致在Tnfb和H2-D基因之间定义了一个新的MHC内重组热点。
