Reeves R H, Rue E E, Citron M P, Cabin D E
Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Genomics. 1997 Jul 15;43(2):202-8. doi: 10.1006/geno.1997.4807.
Five intersubspecific backcrosses and an intercross were used to establish a sex-averaged recombinational map spanning 56 cM across most of mouse Chromosome 16 (Chr 16). A total of 123 markers were ordered using an interval mapping approach to identify 425 recombination sites in a collection of 1154 meioses from 1155 progeny generated in the six crosses. The markers include the 10 "classic" Chr 16 reference markers, 26 additional genes or transcripts including two phenotypic markers (Pit1dw and Kcnj6wv), and 87 simple sequence length polymorphisms (SSLPs). One set of monozygotic twins was detected among the 304 meioses mapped to highest resolution. The reference markers and SSLPs allow the map to be well integrated with existing maps of Chr 16. The average distance between crossover sites is less than 500 kb for most chromosomes, making this collection of recombinant chromosomes useful as a binning and ordering resource for YAC-based physical map assembly on Chr 16.
利用五次种间回交和一次杂交构建了一个性别平均重组图谱，该图谱跨越小鼠第16号染色体（Chr 16）大部分区域，长度为56厘摩。使用区间作图法对总共123个标记进行排序，以在来自六个杂交产生的1155个后代的1154个减数分裂集合中鉴定出425个重组位点。这些标记包括10个“经典”Chr 16参考标记、26个额外的基因或转录本（包括两个表型标记Pit1dw和Kcnj6wv）以及87个简单序列长度多态性（SSLP）。在定位到最高分辨率的304个减数分裂中检测到一组同卵双胞胎。参考标记和SSLP使该图谱能够与Chr 16的现有图谱很好地整合。对于大多数染色体，交叉位点之间的平均距离小于500 kb，这使得这组重组染色体可作为基于酵母人工染色体（YAC）的Chr 16物理图谱组装的分箱和排序资源。
