Assignment of the mouse homologues of 6 loci from HSA1p to chromosomes 3 and 4.

To increase the number of markers on distal mouse chromosome 4, knowledge of the synteny homology between this region and human chromosome 1p (HSA1p) was used to identify candidate homologous mouse genes. Ten probes corresponding to loci on human chromosome 1p were tested to reveal polymorphisms between C57BL/6 and DBA/2 mice, the progenitors of the 26 BXD recombinant inbred strains. These strains were typed with six of these probes and typed for inheritance of mouse-specific microsatellite markers. Five mouse homologues of human genes were assigned to distal mouse chromosome 4 in the following order (including MIT microsatellites): (cen)-D4Mit71-Lap18-D4Mit69-C1qB-Plod/T nfr2-Cd30-(tel). Furthermore, an additional HSA1p marker, Cd53, mapped close to Amy1 on mouse chromosome 3, and a sequence related to Lap18 mapped to mouse chromosome 17, near D17Mit3. This comparative approach suggests that the human counterparts of these genes may have a similar order on human chromosome 1p and also indicates that Lap18, C1qB, and Nhe1 are candidates for a recently described diabetes susceptibility gene on mouse chromosome 4.