小鼠低密度脂蛋白受体相关蛋白/α2-巨球蛋白受体基因的基因组克隆

Genomic cloning of the mouse LDL receptor related protein/alpha 2-macroglobulin receptor gene.

作者信息

van der Zee A, Stas L, Hilleker C, van Leuven F, van Dijk K W, Havekes L, Frants R, Hofker M

机构信息

MGC-Department of Human Genetics, Leiden University, The Netherlands.

出版信息

Genomics. 1994 Sep 1;23(1):256-9. doi: 10.1006/geno.1994.1489.

DOI:10.1006/geno.1994.1489

PMID:7829084

Abstract

The LDL receptor-related protein (LRP) or alpha 2-macroglobulin receptor (A2mr) is encoded by a 15-kb mRNA in mouse and human. Probes encompassing different regions of the mouse cDNA were used to isolate clones from a cosmid library of mouse strain 129. Four overlapping cosmids were used for restriction mapping and Southern blot analysis. This map and hybridization data obtained with oligonucleotide probes from the 5' and 3' ends of the Lrp cDNA demonstrated that the mouse gene is approximately 85 kb in size. The Lrp promoter region was sequenced and reveals strong evolutionary conservation of putative regulatory elements between mouse and human. The present study will facilitate detailed elucidation of the function of LRP in vivo.

摘要

低密度脂蛋白受体相关蛋白（LRP）或α2-巨球蛋白受体（A2mr）在小鼠和人类中由一个15kb的mRNA编码。包含小鼠cDNA不同区域的探针被用于从小鼠129品系的黏粒文库中分离克隆。四个重叠的黏粒用于限制性图谱分析和Southern印迹分析。该图谱以及用来自Lrp cDNA 5'和3'末端的寡核苷酸探针获得的杂交数据表明，小鼠基因大小约为85kb。对Lrp启动子区域进行了测序，结果显示小鼠和人类之间假定调控元件具有很强的进化保守性。本研究将有助于详细阐明LRP在体内的功能。