Wang K, Klotz J L, Kiser G, Bristol G, Hays E, Lai E, Gese E, Kronenberg M, Hood L
Division of Biology, California Institute of Technology, Pasadena 91125.
Genomics. 1994 Apr;20(3):419-28. doi: 10.1006/geno.1994.1196.
The mouse T-cell receptor (TCR) alpha/delta locus was mapped using 17 V alpha and 4 V beta subfamily-specific probes. Four complementary methods were used: (1) an estimate of the V gene repertoire by Southern blot analysis of genomic DNA with subfamily-specific probes; (2) an analysis of V gene segments deleted by TCR gene rearrangements from a panel of T-cell tumors and hybridomas; (3) an analysis of overlapping clusters of cosmid clones; and (4) an analysis of large DNA fragments separated by field-inversion gel electrophoresis. The alpha/delta locus spans about 1 Mb. The distance between the 3'-most V gene segment (V delta 1) and the delta constant gene (C delta) is no more than 150 kb. Sixty-six V gene segments have been mapped physically on cosmids. The members of individual V alpha gene segment subfamilies are dispersed throughout the locus. In contrast, the V delta gene segments V delta 1 to 5 are clustered at the 3' end of the V gene segment cluster. At least two DNA segment duplications, 45 to 80 kb in length, are present in the locus. These data provide information on the evolution of the alpha/delta locus and on organization features that might influence the expression of specific V gene segments in gamma delta cells.
利用17个Vα和4个Vβ亚家族特异性探针绘制了小鼠T细胞受体（TCR）α/δ基因座图谱。采用了四种互补方法：（1）用亚家族特异性探针通过基因组DNA的Southern印迹分析来估计V基因库；（2）分析一组T细胞肿瘤和杂交瘤中因TCR基因重排而缺失的V基因片段；（3）分析黏粒克隆的重叠簇；（4）分析通过场反转凝胶电泳分离的大DNA片段。α/δ基因座跨度约为1 Mb。最3'端的V基因片段（Vδ1）与δ恒定基因（Cδ）之间的距离不超过150 kb。66个V基因片段已在黏粒上进行了物理定位。各个Vα基因片段亚家族的成员分散于整个基因座。相比之下，Vδ基因片段Vδ1至Vδ5聚集在V基因片段簇的3'端。该基因座中存在至少两个长度为45至80 kb的DNA片段重复。这些数据提供了有关α/δ基因座进化以及可能影响γδ细胞中特定V基因片段表达的组织特征的信息。
