抗Hu抗体的核定位与体外细胞毒性无关。

Nuclear localization of anti-Hu antibody is not associated with in vitro cytotoxicity.

作者信息

Hormigo A, Lieberman F

机构信息

Department of Neurology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.

出版信息

J Neuroimmunol. 1994 Dec;55(2):205-12. doi: 10.1016/0165-5728(94)90011-6.

DOI:10.1016/0165-5728(94)90011-6

PMID:7829670

Abstract

IgG fractions of sera containing anti-Hu antibodies or control sera were incubated with Hu-positive and Hu-negative cell lines. Anti-Hu IgG specifically localized in the nucleus of Hu-positive cells. Anti-Hu-positive and control sera were incubated with Hu-positive cells and human complement or peripheral blood mononuclear cells. Hu antibody caused neither complement-mediated lysis nor augmented antibody-dependent cell-mediated cytotoxicity. Anti-Hu IgG did not affect proliferation of Hu-positive cells. Anti-Hu antibodies may not play a direct role in tissue injury in patients with paraneoplastic encephalomyeloneuropathy and anti-Hu antibodies.

摘要

将含有抗Hu抗体的血清IgG组分或对照血清与Hu阳性和Hu阴性细胞系一起孵育。抗Hu IgG特异性定位于Hu阳性细胞的细胞核中。将抗Hu阳性血清和对照血清与Hu阳性细胞以及人补体或外周血单核细胞一起孵育。Hu抗体既不引起补体介导的细胞溶解，也不增强抗体依赖性细胞介导的细胞毒性。抗Hu IgG不影响Hu阳性细胞的增殖。抗Hu抗体可能在副肿瘤性脑脊髓神经病和抗Hu抗体患者的组织损伤中不发挥直接作用。