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[RecF重组途径的遗传控制。II. recL和uvrE突变的影响]

[Genetic control of the RecF recombination pathway. II. Effect of recL- and uvrE- mutations].

作者信息

Smirnov G B, Abdukhalykova G F

出版信息

Genetika. 1976;12(4):100-8.

PMID:783010
Abstract

It was found earlier that the effect of the uvrE- mutation on the yield of recombinants formed via RecF pathway of recombination was mainly due to the decreased viability of the recB-C-sbcB-uvrE- recipient. The results of the present paper show the uvrE- and recL- mutations reduce the recipient ability of the recB-C- sbcB- strains to the same extent. We were unable to find any evidence for complementation between the recL- and uvrE- mutations in transient as well as in stable uvrE-/recL- merozygotes. The recL152 as well as the uvrE502 mutation is lethal for the strains deficient in DNA polymerase I. Both recL- and uvrE- mutations reduce in the recB-C- sbcB- strains the probability of inheritance of the proximal non-selective donor markers as well as selective markers located close to the leading end of the donor chromosome. The former effect however could not be completely due to the latter.

摘要

早期发现,uvrE-突变对通过RecF重组途径形成的重组体产量的影响主要是由于recB-C-sbcB-uvrE-受体菌的生存能力下降。本文结果表明,uvrE-和recL-突变对recB-C-sbcB-菌株受体能力的降低程度相同。在瞬时和稳定的uvrE-/recL-部分二倍体中,我们均未找到recL-和uvrE-突变之间互补的任何证据。recL152以及uvrE502突变对缺乏DNA聚合酶I的菌株是致死的。recL-和uvrE-突变均降低了recB-C-sbcB-菌株中近端非选择性供体标记以及位于供体染色体前端附近的选择性标记的遗传概率。然而,前一种效应不可能完全归因于后一种效应。

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1
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Genetika. 1976;12(4):100-8.
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引用本文的文献

1
Complementation studies with the repair-deficient uvrD3, uvrE156, and recL152 mutations in Escherichia coli.在大肠杆菌中对修复缺陷型uvrD3、uvrE156和recL152突变进行的互补研究。
Mol Gen Genet. 1981;184(3):526-30. doi: 10.1007/BF00352533.
2
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱,第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
3
Molecular cloning of the uvrD gene of Escherichia coli that controls ultraviolet sensitivity and spontaneous mutation frequency.控制紫外线敏感性和自发突变频率的大肠杆菌uvrD基因的分子克隆。
Mol Gen Genet. 1981;184(2):191-9. doi: 10.1007/BF00272904.
4
The effect of inhibition of protein synthesis on UV-irradiated Escherichia coli uvrE cells.
Folia Microbiol (Praha). 1985;30(1):3-16. doi: 10.1007/BF02922491.