Shinohara A, Chiba M, Inaba Y
Department of Epidemiology and Environmental Health, Juntendo University School of Medicine, Tokyo, Japan.
Nihon Eiseigaku Zasshi. 1994 Dec;49(5):924-34. doi: 10.1265/jjh.49.924.
Analytical conditions have been established for determination of trace elements in biological materials by microwave induced plasma-mass spectrometry (MIP-MS). Possible elemental contaminants were checked in the water and reagents used, and during the wet-ashing process. Among 72 elements tested, contamination by Na, Mg, K, Ca, Fe, Ba, and Pb were observed. This contamination was estimated to occur mainly during the process of preparing samples due to the water, reagents and surroundings. Contamination by Ca, Mg, Zn and Pb from tubes for storage was also observed. Adequate conditions for multielement analyses in plasma and bone samples were evaluated. Both plasma and bone samples were digested by the wet-ashing technique before applying MIP-MS. The recovery rates of elements added were decreased depending on the contents of plasma or bone samples in the measuring solutions. The interfering effects of matrix modification due to organs were improved by correction with an internal standard. Recovery rates of elements added, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Rb, Sr, Mo, Cd, Sn, Ba, Pb and fourteen lanthanide elements (La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, and Lu) were within 100 +/- 5% in analytical samples containing 1% volume of human plasma when Ga or Tl was used as the internal standard. Recoveries of Sn and Zn in the bone samples containing 0.1% bone as the original bone material, however, were 100 +/- 10% even after correction by the internal standard, suggesting the necessity of combined use of standard addition methods. The concentrations of V, Cr, Mn, Fe, Cu, Zn, Rb, Sr, Mg, and Ca in plasma from two healthy women were determined by MIP-MS. The data were consistent with the values reported elsewhere, and agreed very closely with those obtained by atomic absorption spectrometry. The accuracy of the values obtained by this method was confirmed using standard reference materials. These results indicate that MIP-MS is a useful method for multielement determination of biological materials.
已经建立了通过微波诱导等离子体质谱法（MIP-MS）测定生物材料中微量元素的分析条件。对所用的水、试剂以及湿灰化过程中可能存在的元素污染物进行了检查。在测试的72种元素中，观察到了钠、镁、钾、钙、铁、钡和铅的污染。据估计，这种污染主要发生在样品制备过程中，原因是水、试剂和环境。还观察到储存管中的钙、镁、锌和铅造成的污染。评估了血浆和骨样品中多元素分析的适当条件。在应用MIP-MS之前，血浆和骨样品均通过湿灰化技术进行消化。添加元素的回收率根据测量溶液中血浆或骨样品的含量而降低。通过内标校正改善了由于器官导致的基质改性的干扰效应。当使用镓或铊作为内标时，在含有1%体积人血浆的分析样品中，添加元素钛、钒、铬、锰、铁、钴、镍、铜、锌、铷、锶、钼、镉、锡、钡、铅和十四种镧系元素（镧、铈、镨、钕、钐、铕、钆、铽、镝、钬、铒、铥、镱和镥）的回收率在100±5%以内。然而，在以0.1%骨作为原始骨材料的骨样品中，即使经过内标校正，锡和锌的回收率仍为100±10%，这表明有必要联合使用标准加入法。通过MIP-MS测定了两名健康女性血浆中钒、铬、锰、铁、铜、锌、铷、锶、镁和钙的浓度。数据与其他地方报道的值一致，并且与通过原子吸收光谱法获得的值非常接近。使用标准参考物质证实了通过该方法获得的值的准确性。这些结果表明，MIP-MS是一种用于生物材料多元素测定的有用方法。
