膳食胆固醇对大鼠肝脏脂肪酶活性及mRNA水平的影响。

Effect of dietary cholesterol on activity and mRNA levels of hepatic lipase in rat.

作者信息

Sultan F, Benhizia F, Lagrange D, Will H, Griglio S

机构信息

B. BRAUN, Medical S.A., Boulogne, France.

出版信息

Life Sci. 1995;56(1):31-7. doi: 10.1016/0024-3205(94)00404-g.

DOI:10.1016/0024-3205(94)00404-g

PMID:7830494

Abstract

Female lean Zucker rats were fed for four weeks with either a control diet or the same diet enriched with 2% (w/w) cholesterol and cholic acid (0.5%, w/w). This treatment resulted in a 6-fold increase in plasma total cholesterol. A 30% decrease was observed in plasma post-heparin HL activity, in contrast with lipoprotein lipase, which was unmodified in the cholesterol/cholate-fed rats. HL activity measured in liver homogenate from these rats was also decreased (-30%, p < 0.05), as was its protein mass, quantified by immunoblot analysis (-57%, (p < 0.01), whereas HL mRNA levels were 3-fold lower in the cholesterol/cholate-fed rats. We conclude that the cholesterol/cholate-enriched diet decreases the HL gene expression by acting at the transcriptional level and/or by affecting HL mRNA stability, or both.

摘要

将雌性瘦素 Zucker 大鼠用对照饮食或添加 2%（w/w）胆固醇和 0.5%（w/w）胆酸的相同饮食喂养四周。这种处理导致血浆总胆固醇增加了 6 倍。与脂蛋白脂肪酶不同，在喂食胆固醇/胆酸盐的大鼠中其未发生改变，而血浆肝素后 HL 活性观察到降低了 30%。这些大鼠肝脏匀浆中测得的 HL 活性也降低了（-30%，p < 0.05），其蛋白质质量也是如此，通过免疫印迹分析定量显示降低了 57%（p < 0.01），而在喂食胆固醇/胆酸盐的大鼠中 HL mRNA 水平降低了 3 倍。我们得出结论，富含胆固醇/胆酸盐的饮食通过在转录水平起作用和/或影响 HL mRNA 稳定性，或两者兼而有之，从而降低 HL 基因表达。