Bénard L, Philippe C, Dondon L, Grunberg-Manago M, Ehresmann B, Ehresmann C, Portier C
Institut de Biologie Physico-Chimique, Paris, France.
Mol Microbiol. 1994 Oct;14(1):31-40. doi: 10.1111/j.1365-2958.1994.tb01264.x.
Expression of rpsO, the gene encoding the small ribosomal protein S15, is autoregulated at the translational level by S15, which binds to its mRNA in a region overlapping the ribosome-binding site. By measuring the effect of mutations on the expression of a translational rpsO-lacZ fusion and the S15 binding affinity for the translational operator, the formation of a pseudoknot in the operator site in vivo is fully demonstrated and appears to be a prerequisite for S15 binding. The mutational analysis suggests also that specific determinants for S15 binding are located in very limited regions of the structure formed by the pseudoknot. It is deduced that a specific pseudoknot conformation is a key element for autoregulation.
编码小核糖体蛋白S15的基因rpsO的表达在翻译水平上受到S15的自动调节，S15与其mRNA在核糖体结合位点重叠的区域结合。通过测量突变对翻译型rpsO - lacZ融合体表达的影响以及S15对翻译操纵子的结合亲和力，体内操纵子位点假结的形成得到了充分证明，并且似乎是S15结合的先决条件。突变分析还表明，S15结合的特定决定因素位于假结形成的结构的非常有限的区域。据推断，特定的假结构象是自动调节的关键要素。
