Thorell J I, Jeppsson S, Holmström B
J Clin Endocrinol Metab. 1976 Sep;43(3):708-11. doi: 10.1210/jcem-43-3-708.
A specific radioimmunoassay for LH, which measures plasma LH in the presence of human chorionic gonadotropin (hCG) is described. Rabbits were immunized with highly purified native LH. One of the antisera with a difference in its reactivity against LH and hCG was further purified by affinity chromatography on a column with hCG coupled to Sepharose 4B. The adsorbed antiserum and 125I-LH was used in a double antibody assay. The LH standard (MRC 68/40) efficiently inhibited the binding of 125I-LH, and the standard curve showed a sensitivity of 0.5 ng/ml in the sample. hCG up to 10 000 ng/ml did not inhibit the binding of 125I-LH. The plasma level of LH in pregnant women in the first trimester was low (1.3 +/- 0.1 ng/ml). When LH was measured in fertile or menopausal women with or without stimulation with LH/FSH releasing hormone (LH-RH)X the results agreed to those found with our conventional LH-assay based on antiserum against hCG.
本文描述了一种特异性促黄体生成素（LH）放射免疫测定法，该方法可在人绒毛膜促性腺激素（hCG）存在的情况下测定血浆中的LH。用高度纯化的天然LH免疫兔子。通过在与Sepharose 4B偶联的hCG柱上进行亲和层析，进一步纯化了一种对LH和hCG反应性不同的抗血清。吸附的抗血清和125I-LH用于双抗体测定。LH标准品（MRC 68/40）有效地抑制了125I-LH的结合，标准曲线显示样品中的灵敏度为0.5 ng/ml。高达10000 ng/ml的hCG并未抑制125I-LH的结合。孕早期孕妇的血浆LH水平较低（1.3±0.1 ng/ml）。当在有或没有促黄体生成素/促卵泡激素释放激素（LH-RH）刺激的育龄或绝经妇女中测量LH时，结果与我们基于抗hCG抗血清的传统LH测定法的结果一致。
