Vandalem J L, Pirens G, Hennen G
Ann Endocrinol (Paris). 1975 Mar-Apr;36(2):117-8.
Antisera directed against the beta subunit of human Luteinizing Hormone (h. LH) and Chorionic Gonadotropin (HCG) were made totally specific to either gonadotropin by adsorbing cross-reacting antibodies. Simple absorption in solution or immunosorption on solid phase were employed. Pituitary functions were examined during pregnancy and until three weeks following delivery h.LH and HCG were assayed before and after injection of Luteinizing Hormone Releasing Hormone (LH-RH). Similiarly TSH was assayed in a homologous TSH beta subunit system before and after injection Thyrotropin Releasing Hormone (TRH). No pituitary release of LH was observed after LH-RH in our experimental conditions while TRH induced a surge of pituitary TSH in the blood.
通过吸附交叉反应抗体，制备了针对人促黄体生成素（h.LH）和绒毛膜促性腺激素（HCG）β亚基的抗血清，使其对任一促性腺激素具有完全特异性。采用溶液中的简单吸附或固相免疫吸附。在怀孕期间检查垂体功能，直到分娩后三周，在注射促黄体生成素释放激素（LH-RH）前后测定h.LH和HCG。同样，在注射促甲状腺激素释放激素（TRH）前后，在同源促甲状腺激素β亚基系统中测定促甲状腺激素（TSH）。在我们的实验条件下，LH-RH注射后未观察到垂体释放LH，而TRH诱导血液中垂体TSH激增。
