Sato C, Hirota K, Matsumoto G
Electrotechnical Laboratory, Supermolecular Science Division, Ibaraki, Japan.
Biochem Biophys Res Commun. 1995 Jan 26;206(3):807-13. doi: 10.1006/bbrc.1995.1115.
The distribution of SQSC1 mRNA in tissues of squid Loligo bleekeri was studied by the blot hybridization method. The complete cDNA for the coding region of SQSC1, the invertebrate putative sodium channel, was prepared from squid optic lobe (Sato and Matsumoto, Biochem. Biophys. Res. Comm. 186, 61-68, 1992). Transcriptional products of the SQSC1 gene were found to consist of two main different lengths (12 and 9 kb). The transcriptional products were detected in all the nervous tissues examined: optic lobes, cerebral ganglia and giant stellate ganglia. However, it was not detected in the muscle, suggesting the SQSC1 gene is specific for sodium channels of squid nerve cells. SQSC1 appears more widely distributed in the nervous system than GFLN1 which they reported as expressed specifically in stellate ganglion of the squid (Rosenthal and Gilly, Proc. Natl. Acad. Sci. USA 90, 10026-10030, 1993).
采用印迹杂交法研究了剑尖枪乌贼（Loligo bleekeri）组织中SQSC1 mRNA的分布情况。无脊椎动物假定的钠通道SQSC1编码区的完整cDNA是从枪乌贼视叶中制备的（佐藤和松本，《生物化学与生物物理研究通讯》186, 61 - 68, 1992）。发现SQSC1基因的转录产物由两种主要的不同长度（12 kb和9 kb）组成。在所检测的所有神经组织中均检测到了转录产物：视叶、脑神经节和巨大星状神经节。然而，在肌肉中未检测到，这表明SQSC1基因对枪乌贼神经细胞的钠通道具有特异性。与他们报道的在枪乌贼星状神经节中特异性表达的GFLN1相比，SQSC1在神经系统中的分布似乎更广泛（罗森塔尔和吉利，《美国国家科学院院刊》90, 10026 - 10030, 1993）。
